Journal: Frontiers in Oncology
Article Title: The prognostic value of immune-related genes AZGP1 , SLCO5A1 , and CTF1 in Uveal melanoma
doi: 10.3389/fonc.2022.918230
Figure Lengend Snippet: Verification of the expression of the three core genes in cells (A–C) The expression of AZGP1, SLCO5A1, and CTF1 in Human retinal pigment epithelial cell line(hTERT RPE-1) and uveal melanoma cells (OM431) was detected by RT-qPCR, cellular experiments were performed in three independent replicates, data in the figures are all measures in the form of mean ± standard deviation, data were analyzed by t-test,** indicates p < 0.01.
Article Snippet: Human UM cell line OM431 and normal UM cells changed to: Human retinal pigment epithelial cell line (hTERT RPE-1 U1L) were purchased from ATCC (American Type Culture Collection) and cultured in DMEM (Dulbecco's Modified Eagle Medium) containing 100 ml/L fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C with 5% CO 2 by volume.
Techniques: Expressing, Quantitative RT-PCR, Standard Deviation